The Sec61A Monoclonal Antibody (CAB19795) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced using rabbits as hosts, is highly specific to human SEC61A and is validated for use in Western blot applications.SEC61A is essential for the proper functioning of the protein translocation machinery, making it a key target for investigating cellular processes related to protein synthesis, folding, and trafficking. By binding to SEC61A, this antibody enables researchers to detect and study the expression levels and localization of SEC61A in various cell types, aiding in the understanding of its role in cellular function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Sec61A Monoclonal Antibody
SKU:
CAB19795
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2323
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Sec61 alpha-2, SEC61A2, Sec61A
Positive Sample:
293T, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain, Rat testis
Cellular Localization:
Endoplasmic Reticulum Membrane.
Calculated MW:
52kDa
Observed MW:
52kDa
The protein encoded by this gene has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. It may also be required for the assembly of membrane and secretory proteins. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55176
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Sec61A Rabbit mAb (CAB19795) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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