Developmental Biology

Anti-SCRIB Antibody (CAB17450)

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SKU:
CAB17450
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-SCRIB Antibody
Antibody SKU:CAB17450
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human SCRIB.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, 293T
Immunogen:Recombinant protein of human SCRIB.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:23513
Uniprot:Q14160
Cellular Location:
Calculated MW:175kDa
Observed MW:260kDa
Synonyms:CRIB1, SCRB1, SCRIB1, Vartul, SCRIB
Background:This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
UniProt Protein Function:Scribble: may be involved in regulating neuroblast cortical polarity, cell size asymmetry and mitotic spindle asymmetry. A neoplastic tumor suppressor genes in Drosophila melanogaster. May play a role in the suppression of mammalian tumorigenesis. Gene ablation in the mouse causes severe neural tube defects. May be implicated in the etiology of human NTDs. Involved in the regulation of synaptic plasticity and synaptic vesicle dynamics. May be required to sustain synaptic vesicle concentrations at their sites of release. Targeted for ubiquitin-mediated degradation by the HPV E6 oncoprotein.
UniProt Protein Details:

Protein type:Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cell-cell adherens junction; cytoplasm; intercellular junction; leading edge; nucleoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane

Molecular Function:protein binding

Biological Process: cell migration; cell proliferation; cell-cell adhesion; establishment of apical/basal cell polarity; negative regulation of mitotic cell cycle; neural tube closure; positive chemotaxis; positive regulation of apoptosis; positive regulation of receptor recycling

NCBI Summary:This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:Q14160
NCBI GenInfo Identifier:261260101
NCBI Gene ID:23513
NCBI Accession:Q14160.4
UniProt Secondary Accession:Q14160,Q6P496, Q7Z5D1, Q8WWV8, Q96C69, Q96GG1,
UniProt Related Accession:Q14160
Molecular Weight:177,694 Da
NCBI Full Name:Protein scribble homolog
NCBI Synonym Full Names:scribbled planar cell polarity protein
NCBI Official Symbol:SCRIB  
NCBI Official Synonym Symbols:CRIB1; SCRB1; SCRIB1; Vartul  
NCBI Protein Information:protein scribble homolog
UniProt Protein Name:Protein scribble homolog
UniProt Synonym Protein Names:Protein LAP4
Protein Family:Protein
UniProt Gene Name:SCRIB  
UniProt Entry Name:SCRIB_HUMAN
Anti-SCRIB Antibody (CAB17450)Western blot analysis of extracts of various cell lines, using SCRIB antibody (CAB17450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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