KO Validated Antibodies 1

Anti-S100A11 Antibody (CAB5486)[KO Validated]

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SKU:
CAB5486
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-S100A11 Antibody [KO Validated]
Antibody SKU:CAB5486
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human
Positive Samples:BxPC-3, BT-474, LOVO, 22Rv1, SKOV3, THP-1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAKI SSPT ETER CIES LIAV FQKY AGKD GYNY TLSK TEFL SFMN TELA AFTK NQKD PGVL DRMM KKLD TNSD GQLD FSEF LNLI GGLA MACH DSFL KAVP SQKR T
Gene ID:6282
Uniprot:P31949
Cellular Location:Cytoplasm, Nucleus
Calculated MW:11kDa
Observed MW:12kDa
Synonyms:S100A11, HEL-S-43, MLN70, S100C
Background:The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
UniProt Protein Function:S100A11: a calcium-binding regulatory protein of the S-100 family. Interacts with the N-termini of annexins A1. May regulate fusion processes such as endo- and exocytosis. Increases in the early stage of pancreatic carcinogenesis and decreases during subsequent progression to cancer. Increased expression in anaplastic large cell lymphoma.
UniProt Protein Details:

Protein type:Calcium-binding; DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; extracellular space; nucleus; ruffle

Molecular Function:calcium-dependent protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of cell proliferation; negative regulation of DNA replication; signal transduction

NCBI Summary:The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008]
UniProt Code:P31949
NCBI GenInfo Identifier:1710818
NCBI Gene ID:6282
NCBI Accession:P31949.2
UniProt Secondary Accession:P31949,Q5VTK0,
UniProt Related Accession:P31949
Molecular Weight:11,740 Da
NCBI Full Name:Protein S100-A11
NCBI Synonym Full Names:S100 calcium binding protein A11
NCBI Official Symbol:S100A11  
NCBI Official Synonym Symbols:MLN70; S100C; HEL-S-43  
NCBI Protein Information:protein S100-A11
UniProt Protein Name:Protein S100-A11
UniProt Synonym Protein Names:Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70
Protein Family:Protein
UniProt Gene Name:S100A11  
UniProt Entry Name:S10AB_HUMAN
Anti-S100A11 Antibody [KO Validated] (CAB5486)Western blot analysis of extracts of various cell lines, using S100A11 antibody (CAB5486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-S100A11 Antibody [KO Validated] (CAB5486)Western blot analysis of extracts from normal (control) and S100A11 knockout (KO) HeLa cells, using S100A11 antibody (CAB5486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-S100A11 Antibody [KO Validated] (CAB5486)Immunohistochemistry of paraffin-embedded human kidney using S100A11 antibody (CAB5486) at dilution of 1:100 (40x lens).
Anti-S100A11 Antibody [KO Validated] (CAB5486)Immunofluorescence analysis of U2OS cells using S100A11 antibody (CAB5486). Blue: DAPI for nuclear staining.
Anti-S100A11 Antibody [KO Validated] (CAB5486)Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug S100A11 antibody (CAB5486). Western blot was performed from the immunoprecipitate using S100A11 antibody (CAB5486) at a dilition of 1:1000.
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