Epigenetics & Nuclear Signaling Antibodies 1

Anti-RUVBL2 Antibody (CAB12564)

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SKU:
CAB12564
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RUVBL2 Antibody
Antibody SKU:CAB12564
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human RUVBL2 (NP_006657.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, K-562, SW480, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human RUVBL2 (NP_006657.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATV TATT KVPE IRDV TRIE RIGA HSHI RGLG LDDA LEPR QASQ GMVG QLAA RRAA GVVL EMIR EGKI AGRA VLIA GQPG TGKT AIAM GMAQ ALGP DTPF TAIA GSEI FSLE MSKT EALT QAFR RSIG VRIK EETE IIEG EVVE IQID RPAT GTGS KVGK LTLK TTEM ETIY DLGT KMIE SLTK DKVQ AGDV ITID KATG KISK LGRS FTRA RDYD AMGS QTKF VQCP DGEL QKRK EVVH TVSL HEID VINS RTQG FLAL FSGD TGEI KSEV REQI NAKV AEWR EEGK AEII PGVL FIDE VHML DIES FSFL NRAL ESDM APVL IMAT NRGI TRIR GTSY QSPH GIPI DLLD RLLI VSTT PYSE KDTK QILR IRCE EEDV EMSE DAYT VLTR IGLE TSLR YAIQ LITA ASLV CRKR KGTE VQVD DIKR VYSL FLDE SRST QYMK EYQD AFLF NELK GETM DTS
Gene ID:10856
Uniprot:Q9Y230
Cellular Location:Cytoplasm, Membrane, Nucleus, Nucleus matrix, nucleoplasm
Calculated MW:46kDa/51kDa
Observed MW:51kDa
Synonyms:RUVBL2, CGI-46, ECP-51, ECP51, INO80J, REPTIN, RVB2, TAP54-beta, TIH2, TIP48, TIP49B
Background:This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
UniProt Protein Function:RUVBL2: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin- remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C- terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Ubiquitously expressed. Highly expressed in testis and thymus. Belongs to the RuvB family.
UniProt Protein Details:

Protein type:EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear matrix; membrane; cytoplasm; intracellular; nucleus; ribonucleoprotein complex

Molecular Function:ATP-dependent DNA helicase activity; identical protein binding; protein binding; DNA helicase activity; chromatin DNA binding; ATP-dependent 5'-3' DNA helicase activity; unfolded protein binding; ATPase activity; damaged DNA binding; ATP binding

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; protein folding; transcription, DNA-dependent; regulation of growth; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; DNA repair; DNA duplex unwinding; DNA recombination

NCBI Summary:This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y230
NCBI GenInfo Identifier:28201890
NCBI Gene ID:10856
NCBI Accession:Q9Y230.3
UniProt Secondary Accession:Q9Y230,Q6FIB9, Q6PK27, Q9Y361, B3KQ59, E7ETE5,
UniProt Related Accession:Q9Y230
Molecular Weight:463
NCBI Full Name:RuvB-like 2
NCBI Synonym Full Names:RuvB-like AAA ATPase 2
NCBI Official Symbol:RUVBL2  
NCBI Official Synonym Symbols:RVB2; TIH2; ECP51; TIP48; CGI-46; INO80J; REPTIN; TIP49B  
NCBI Protein Information:ruvB-like 2; ECP-51; TAP54-beta; reptin52 protein; repressing pontin 52; INO80 complex subunit J; RuvB (E coli homolog)-like 2; 48 kDa TBP-interacting protein; TIP60-associated protein 54-beta; 51 kDa erythrocyte cytosolic protein; erythrocyte cytosolic protein, 51-KD; 48 kDa TATA box-binding protein-interacting protein
UniProt Protein Name:RuvB-like 2
UniProt Synonym Protein Names:48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; INO80 complex subunit J; Repressing pontin 52; Reptin 52; TIP49b; TIP60-associated protein 54-beta; TAP54-beta
Protein Family:RuvB
UniProt Gene Name:RUVBL2  
UniProt Entry Name:RUVB2_HUMAN
Anti-RUVBL2 Antibody (CAB12564)Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (CAB12564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-RUVBL2 Antibody (CAB12564)Immunohistochemistry of paraffin-embedded rat lung using RUVBL2 antibody (CAB12564) at dilution of 1:100 (40x lens).
Anti-RUVBL2 Antibody (CAB12564)Immunohistochemistry of paraffin-embedded mouse brain using RUVBL2 antibody (CAB12564) at dilution of 1:100 (40x lens).
Anti-RUVBL2 Antibody (CAB12564)Immunofluorescence analysis of MCF-7 cells using RUVBL2 antibody (CAB12564). Blue: DAPI for nuclear staining.
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