Epigenetics & Nuclear Signaling Antibodies 5
Anti-RRM1 Antibody (CAB4259)
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- SKU:
- CAB4259
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RRM1 Antibody |
| Antibody SKU: | CAB4259 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human RRM1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, Raji, Mouse testis, Rat lung |
| Immunogen: | A synthesized peptide derived from human RRM1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6240 |
| Uniprot: | P23921 |
| Cellular Location: | |
| Calculated MW: | 90kDa |
| Observed MW: | 90KDa |
| Synonyms: | R1, RIR1, RR1 |
| Background: | This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| UniProt Protein Function: | RRM1: an enzyme involved in DNA replication that provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Belongs to the ribonucleoside diphosphate reductase large chain family. Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the M1 subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site. Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. The level of the enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Patients with advanced non-small cell lung cancer responded favorably to gemcitabine. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Other Amino Acids Metabolism - glutathione; DNA replication; EC 1.17.4.1; Nucleotide Metabolism - pyrimidine; Oxidoreductase Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: nucleoplasm; cell projection; cell soma; cytoplasm; nuclear envelope; cytosol Molecular Function:protein binding; ATP binding; ribonucleoside-diphosphate reductase activity Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; retina development in camera-type eye; cell proliferation in forebrain; male gonad development; deoxyribonucleotide biosynthetic process; protein heterotetramerization; mitotic cell cycle; response to ionizing radiation; DNA replication |
| NCBI Summary: | This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P23921 |
| NCBI GenInfo Identifier: | 132608 |
| NCBI Gene ID: | 6240 |
| NCBI Accession: | P23921.1 |
| UniProt Secondary Accession: | P23921,P07742, |
| UniProt Related Accession: | P23921 |
| Molecular Weight: | 90kDa |
| NCBI Full Name: | Ribonucleoside-diphosphate reductase large subunit |
| NCBI Synonym Full Names: | ribonucleotide reductase catalytic subunit M1 |
| NCBI Official Symbol: | RRM1 |
| NCBI Official Synonym Symbols: | R1; RR1; RIR1 |
| NCBI Protein Information: | ribonucleoside-diphosphate reductase large subunit |
| UniProt Protein Name: | Ribonucleoside-diphosphate reductase large subunit |
| UniProt Synonym Protein Names: | Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit |
| UniProt Gene Name: | RRM1 |
| UniProt Entry Name: | RIR1_HUMAN |
 | Western blot - RRM1 Rabbit mAb (CAB4259) |
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