Cell Biology Antibodies 10

RPS27 Rabbit Polyclonal Antibody (CAB6729)

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SKU:
CAB6729
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Rat
Synonyms:
S27, MPS1, eS27, DBA17, MPS-1, RPS27
Research Use:
Cell Biology
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Description

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RPS27 Antibody (CAB6729)

The RPS27 Antibody (CAB6729) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for its high reactivity with human samples and is specifically designed for use in Western blot applications.RPS27, also known as Ribosomal Protein S27, plays a crucial role in protein synthesis and cellular growth. It is involved in maintaining the structural integrity of the ribosome and is essential for proper ribosomal function.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:RPS27 Antibody
SKU:CAB6729
Size:20μL, 100μL
Reactivity:Human, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPLA KDLL HPSP EEEK RKHK KKRL VQSP NSYF MDVK CPGC YKIT TVFS HAQT VVLC VGCS TVLC QPTG GKAR LTEG CSFR RKQH
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:S27, MPS1, eS27, DBA17, MPS-1, RPS27
Positive Sample:MCF7, HeLa, H460, Rat liver
Cellular Localization:Cytosol, Cytosolic Ribosome, Nucleoplasm, Nucleus, Postsynaptic Density.
Calculated MW:9kDa
Observed MW:10kDa

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Purification MethodAffinity purification
Gene ID6232
RRIDAB_2767313
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RPS27 Rabbit pAb (CAB6729) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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