RHOG Rabbit Polyclonal Antibody (CAB19302)

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SKU:
CAB19302
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Reactivity:
Human, Mouse, Rat
Synonyms:
ARHG, RHOG
Frequently bought together:

Description

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RHOG Antibody (CAB19302)

The RHOG Antibody (CAB19302) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.RHOG plays a crucial role in signal transduction pathways that control actin dynamics, making it a key player in processes like cell adhesion and migration.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RHOG Antibody
SKU:CAB19302
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:TVNL NLWD TAGQ EEYD RLRT LSYP QTNV FVIC FSIA SPPS YENV RHKW HPEV CHHC PDVP ILLV GTKK DLRA QPDT LRRL KEQG QAPI TPQQ GQAL AKQI H
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ARHG, RHOG
Cellular Localization:Cell Cortex, Cell Projection, Cytoplasmic Vesicle, Cytoskeleton, Cytosol, Extracellular Exosome, Focal Adhesion, Plasma Membrane.
Calculated MW:21kDa
Observed MW:Refertofigures

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X.

Purification MethodAffinity purification
Gene ID391
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RHOG Rabbit pAb (CAB19302) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver cancer using RHOG Rabbit pAb (CAB19302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human appendix using RHOG Rabbit pAb (CAB19302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using RHOG Rabbit pAb (CAB19302) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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