Antibody Name:	Anti-RHOC Antibody [KO Validated]
Antibody SKU:	CAB1062
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U2OS, Jurkat, A-549, PC-12

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAI RKKL VIVG DGAC GKTC LLIV FSKD QFPE VYVP TVFE NYIA DIEV DGKQ VELA LWDT AGQE DYDR LRPL SYPD TDVI LMCF SIDS PDSL ENIP EKWT PEVK HFCP NVPI ILVG NKKD LRQD EHTR RELA KMKQ EPVR SEEG RDMA NRIS AFGY LECS AKTK EGVR EVFE MATR AGLQ VRKN KRRR GCPI L
Gene ID:	389
Uniprot:	P08134
Cellular Location:	Cell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor
Calculated MW:	22kDa
Observed MW:	22kDa

Synonyms:

RHOC, ARH9, ARHC, H9, RHOH9

Background:

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	RHOC: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
UniProt Protein Details:	Protein type:G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: cleavage furrow; cytosol; plasma membrane Molecular Function:protein binding; signal transducer activity Biological Process: apical junction assembly; cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of lipase activity; regulation of small GTPase mediated signal transduction
NCBI Summary:	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P08134
NCBI GenInfo Identifier:	132543
NCBI Gene ID:	389
NCBI Accession:	P08134.1
UniProt Secondary Accession:	P08134,Q6ICN3, B3KSW1,
UniProt Related Accession:	P08134
Molecular Weight:	22,006 Da
NCBI Full Name:	Rho-related GTP-binding protein RhoC
NCBI Synonym Full Names:	ras homolog family member C
NCBI Official Symbol:	RHOC
NCBI Official Synonym Symbols:	H9; ARH9; ARHC; RHOH9
NCBI Protein Information:	rho-related GTP-binding protein RhoC
UniProt Protein Name:	Rho-related GTP-binding protein RhoC
UniProt Synonym Protein Names:	Rho cDNA clone 9; h9
Protein Family:	Rho-related GTP-binding protein
UniProt Gene Name:	RHOC
UniProt Entry Name:	RHOC_HUMAN

	Western blot analysis of extracts of various cell lines, using RHOC antibody (CAB1062). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and RHOC knockout (KO) 293T cells, using RHOC antibody (CAB1062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 300s.
	Immunofluorescence analysis of HeLa cells using RHOC antibody (CAB1062). Blue: DAPI for nuclear staining.