Epigenetics & Nuclear Signaling Antibodies 3

Anti-RFC4 Antibody (CAB5485)

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SKU:
CAB5485
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RFC4 Antibody
Antibody SKU:CAB5485
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DKIQ QQRL LDIA KKEN VKIS DEGI AYLV KVSE GDLR KAIT FLQS ATRL TGGK EITE KVIT DIAG VIPA EKID GVFA ACQS GSFD KLEA VVKD LIDE GHAA TQLV NQLH DVVV ENNL SDKQ KSII TEKL AEVD KCLA DGAD EHLQ LISL CATV MQQL SQNC
Gene ID:5984
Uniprot:P35249
Cellular Location:Nucleus
Calculated MW:33kDa/39kDa
Observed MW:40kDa
Synonyms:RFC4, A1, RFC37
Background:The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
UniProt Protein Function:RFC4: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Belongs to the activator 1 small subunits family.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: nucleoplasm; DNA replication factor C complex; cytoplasm; nucleus

Molecular Function:protein binding; ATP binding; DNA clamp loader activity

Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; DNA strand elongation during DNA replication; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA repair; telomere maintenance

NCBI Summary:The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:P35249
NCBI GenInfo Identifier:1703052
NCBI Gene ID:5984
NCBI Accession:P35249.2
UniProt Secondary Accession:P35249,Q6FHX7, B4DM41, D3DNV2,
UniProt Related Accession:P35249
Molecular Weight:363
NCBI Full Name:Replication factor C subunit 4
NCBI Synonym Full Names:replication factor C (activator 1) 4, 37kDa
NCBI Official Symbol:RFC4  
NCBI Official Synonym Symbols:A1; RFC37  
NCBI Protein Information:replication factor C subunit 4; A1 37 kDa subunit; RFC 37 kDa subunit; RF-C 37 kDa subunit; activator 1 subunit 4; activator 1 37 kDa subunit; replication factor C 37 kDa subunit
UniProt Protein Name:Replication factor C subunit 4
UniProt Synonym Protein Names:Activator 1 37 kDa subunit; A1 37 kDa subunit; Activator 1 subunit 4; Replication factor C 37 kDa subunit; RF-C 37 kDa subunit; RFC37
Protein Family:Replication factor
UniProt Gene Name:RFC4  
UniProt Entry Name:RFC4_HUMAN
Anti-RFC4 Antibody (CAB5485)Western blot analysis of extracts of various cell lines, using RFC4 antibody (CAB5485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RFC4 Antibody (CAB5485)Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens).
Anti-RFC4 Antibody (CAB5485)Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens).
Anti-RFC4 Antibody (CAB5485)Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens).
Anti-RFC4 Antibody (CAB5485)Immunofluorescence analysis of U2OS cells using RFC4 antibody (CAB5485). Blue: DAPI for nuclear staining.
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