Epigenetics & Nuclear Signaling Antibodies 4

REST/NRSF Rabbit Polyclonal Antibody (CAB7161)

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SKU:
CAB7161
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
WT6, XBR, HGF5, NRSF, DFNA27, GINGF5, REST/NRSF
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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REST/NRSF Antibody (CAB7161)

The REST/NRSF Antibody (CAB7161) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human and mouse samples and has been validated for use in various applications including Western blot, immunofluorescence, and immunohistochemistry.REST is known for its role in repressing neuronal genes in non-neuronal cells, making it a crucial factor in neural development and function.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:REST/NRSF Antibody
SKU:CAB7161
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ALPN DMYD LHDL SKAE LAAP QLIM LANV ALTG EVNG SCCD YLVG EERQ MAEL MPVG DNNF SDSE EGEG LEES ADIK GEPH GLEN MELR SLEL SVVE PQPV FEAS GA
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:WT6, XBR, HGF5, NRSF, DFNA27, GINGF5, REST/NRSF
Positive Sample:HeLa, Raji, SH-SY5Y, Mouse heart, Rat heart
Cellular Localization:Nucleus.
Calculated MW:122kDa
Observed MW:150kDa

This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described.

Purification MethodAffinity purification
Gene ID5978
RRIDAB_2767714
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using REST/NRSF Rabbit pAb (CAB7161) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using REST/NRSF Rabbit pAb (CAB7161) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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