Signal Transduction Antibodies 3

RCVRN Rabbit Polyclonal Antibody (CAB6404)

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SKU:
CAB6404
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
RCV1, RCVRN
Research Use:
Signal Transduction
Frequently bought together:

Description

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RCVRN Antibody (CAB6404)

The RCVRN Antibody (CAB6404) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting recoverin in human samples, making it ideal for experiments such as Western blotting.Recoverin is a calcium-binding protein found in the retina, where it is involved in regulating the visual cycle and signaling pathways.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RCVRN Antibody
SKU:CAB6404
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGNS KSGA LSKE ILEE LQLN TKFS EEEL CSWY QSFL KDCP TGRI TQQQ FQSI YAKF FPDT DPKA YAQH VFRS FDSN LDGT LDFK EYVI ALHM TTAG KTNQ KLEW AFSL YDVD GNGT ISKN EVLE IVMA IFKM ITPE DVKL LPDD ENTP EKRA EKIW KYFG KNDD DKLT EKEF IEGT LANK EILR LIQF EPQK VKEK MKNA
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:5000
IF-P1:10 - 1:100
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RCV1, RCVRN
Positive Sample:Mouse eye, Rat eye
Cellular Localization:Dendrite, Perikaryon.
Calculated MW:23kDa
Observed MW:23kDa

This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.

Purification MethodAffinity purification
Gene ID5957
RRIDAB_2767006
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RCVRN Rabbit pAb (CAB6404) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat retina using RCVRN Rabbit pAb (CAB6404) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse eye using RCVRN Rabbit pAb (CAB6404) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of paraffin-embedded rat eye using RCVRN Rabbit pAb (CAB6404) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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