The Rad50 Monoclonal Antibody (CAB3869) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody is raised in rabbits and is suitable for use in a variety of applications, including Western blotting and immunofluorescence. It binds specifically to the RAD50 protein, allowing for precise detection and analysis in a range of cell types.RAD50 is a critical component of the MRN complex, involved in sensing and repairing DNA double-strand breaks. Dysregulation of RAD50 function has been implicated in various genetic disorders and cancer development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rad50 Monoclonal Antibody
SKU:
CAB3869
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0854
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NBSLD, RAD502, hRad50, Rad50
Positive Sample:
HeLa, K-562, U-87MG, Mouse testis, Rat testis
Cellular Localization:
Chromosome, Nucleus, Telomere.
Calculated MW:
154kDa
Observed MW:
154kDa
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Purification Method
Affinity purification
Gene ID
10111
RRID
AB_2863151
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad50 Rabbit mAb (CAB3869) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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