Epigenetics & Nuclear Signaling Antibodies 3

Rad18 Rabbit Polyclonal Antibody (CAB5380)

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SKU:
CAB5380
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Synonyms:
RNF73, Rad18
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Rad18 Antibody (CAB5380)

The Rad18 Antibody (CAB5380) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RAD18 protein, this antibody allows for the accurate detection and analysis of RAD18 in various cell types, making it an excellent choice for those working in the fields of molecular biology and cancer research.RAD18 is known for its role in DNA repair mechanisms, specifically in the process of post-replication repair and DNA damage tolerance.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Rad18 Antibody
SKU:CAB5380
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:INKH LDSC LSRE EKKE SLRS SVHK RKPL PKTV YNLL SDRD LKKK LKEH GLSI QGNK QQLI KRHQ EFVH MYNA QCDA LHPK SAAE IVRE IENI EKTR MRLE ASKL NESV MVFT KDQT EKEI DEIH SKYR KKHK SEFQ LLVD QARK GYKK IAGM SQKT VTIT KEDE STEK LSSV CMGQ EDNM TSVT NHFS QSKL DSPE ELEP DREE DSSS CIDI QEVL SSSE SDSC NSSS SDII RDLL EEEE AWEA SHKN DLQD TEIS PRQN RRTR AAES AEIE PRNK RNRN
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RNF73, Rad18
Positive Sample:MCF7
Cellular Localization:Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:56kDa
Observed MW:80kDa

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Purification MethodAffinity purification
Gene ID56852
RRIDAB_2766190
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from MCF7 cells, using Rad18 Rabbit pAb (CAB5380) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
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