Name: Anti-Rad18 Antibody (CAB4339)
Brand: Epigenetics & Nuclear Signaling Antibodies 5
SKU: CAB4339
Availability: OutOfStock

Antibody Name:	Anti-Rad18 Antibody
Antibody SKU:	CAB4339
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Rad18

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, MCF7, K-562, Mouse testis, Mouse brain, Mouse spleen, Rat testis

Immunogen:	A synthesized peptide derived from human Rad18
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	56852
Uniprot:	Q9NS91
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	56kDa
Observed MW:	70KDa/90KDa

Synonyms:

RNF73

Background:

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	RAD18: a structural maintenance of chromosomes (SMC) protein involved in postreplication repair of UV-damaged DNA. Has ssDNA binding activity. Interacts with Rad6 through a conserved ring-finger motif. Rad6 is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA.
UniProt Protein Details:	Protein type:DNA repair, damage; EC 6.3.2.19; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 3p25.3 Cellular Component: XY body; replication fork; nucleus; chromatin Molecular Function:Y-form DNA binding; protein binding; zinc ion binding; ubiquitin protein ligase binding; damaged DNA binding; polyubiquitin binding; ligase activity Biological Process: negative regulation of DNA recombination; protein ubiquitination; spermatogenesis; DNA repair; response to UV
NCBI Summary:	The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NS91
NCBI GenInfo Identifier:	313104165
NCBI Gene ID:	56852
NCBI Accession:	Q9NS91.2
UniProt Secondary Accession:	Q9NS91,Q58F55, Q9NRT6,
UniProt Related Accession:	Q9NS91
Molecular Weight:	495
NCBI Full Name:	E3 ubiquitin-protein ligase RAD18
NCBI Synonym Full Names:	RAD18 E3 ubiquitin protein ligase
NCBI Official Symbol:	RAD18
NCBI Official Synonym Symbols:	RNF73
NCBI Protein Information:	E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18 homolog; RING finger protein 73; RAD18, S. cerevisiae, homolog; postreplication repair protein RAD18; postreplication repair protein hRAD18p
UniProt Protein Name:	E3 ubiquitin-protein ligase RAD18
UniProt Synonym Protein Names:	Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RAD18
UniProt Entry Name:	RAD18_HUMAN