Autophagy Antibodies

RAB8A Rabbit Polyclonal Antibody (CAB17369)

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SKU:
CAB17369
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
Reactivity:
Human, Mouse, Rat
Synonyms:
MEL, RAB8, RAB8A
Research Use:
Autophagy
Frequently bought together:

Description

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RAB8A Antibody (CAB17369)

The RAB8A Antibody (CAB17369) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting Rab8a in various samples, particularly human cell lines.Rab8a is a member of the Rab GTPase family that plays a pivotal role in regulating vesicular transport and protein trafficking within cells. Its dysregulation has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RAB8A Antibody
SKU:CAB17369
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ALDY GIKF METS AKAN INVE NAFF TLAR DIKA KMDK KLEG NSPQ GSNQ GVKI TPDQ QKRS SFFR CVLL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MEL, RAB8, RAB8A
Positive Sample:HCT116, HeLa, C6
Cellular Localization:Cell Membrane, Cell Projection, Cytoplasm, Cytoplasmic Side, Cytoplasmic Vesicle, Golgi Apparatus, Lipid-Anchor, Recycling Endosome Membrane, Centriole, Centrosome, Cilium, Cilium Basal Body, Cytoskeleton, Microtubule Organizing Center, Phagosome, Phagosome Membrane.
Calculated MW:24kDa
Observed MW:24kDa

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

Purification MethodAffinity purification
Gene ID4218
RRIDAB_2771919
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RAB8A Rabbit pAb (CAB17369) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from C6 cells, using RAB8A Rabbit pAb (CAB17369) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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