Developmental Biology

Pumilio 1 Rabbit Polyclonal Antibody (CAB6108)

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SKU:
CAB6108
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PUMH, HSPUM, PUMH1, PUML1, SCA47, Pumilio 1
Research Use:
Developmental Biology
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Description

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Pumilio 1 Antibody (CAB6108)

The Pumilio 1 Antibody (CAB6108) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications.Pumilio homolog 1 is known to be involved in a variety of biological processes, including embryonic development, cell cycle progression, and stem cell maintenance.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Pumilio 1 Antibody
SKU:CAB6108
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSVA CVLK RKAV LWQD SFSP HLKH HPQE PANP NMPV VLTS GTGS QAQP QPAA NQAL AAGT HSSP VPGS IGVA GRSQ DDAM VDYF FQRQ HGEQ LGGG GSGG GGYN NSKH RWPT GDNI HAEH QVRS MDEL NHDF QALA LEGR AMGE QLLP GKKF WETD ESSK DGPK GIFL GDQW RDSA WGTS DHSV SQPI MVQR RPGQ SFHV NSEV NSVL SPRS ESGG LGVS MVEY VLSS SPGD SCLR KGGF GPRD ADSD ENDK GEKK NKGT FDGD KLGD LKEE GDVM DKTN
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PUMH, HSPUM, PUMH1, PUML1, SCA47, Pumilio 1
Positive Sample:Mouse brain, Rat brain
Cellular Localization:Cytoplasm, Cytoplasmic Granule, P-Body.
Calculated MW:126kDa
Observed MW:135kDa

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.

Purification MethodAffinity purification
Gene ID9698
RRIDAB_2766749
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Pumilio 1 Rabbit pAb (CAB6108) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Product ImageImmunofluorescence analysis of U2OS cells using Pumilio 1 Rabbit pAb (CAB6108) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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