Cell Biology Antibodies 18

Anti-PTRF Antibody (CAB18331)

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SKU:
CAB18331
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PTRF Antibody
Antibody SKU:CAB18331
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PTRF.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, HeLa, A-549, Mouse stomach, Mouse lung, Mouse heart, Rat heart
Immunogen:Recombinant protein of human PTRF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:284119
Uniprot:Q6NZI2
Cellular Location:
Calculated MW:43kDa
Observed MW:55kDa
Anti-PTRF Antibody (CAB18331)Western blot - PTRF Rabbit pAb (CAB18331)
Synonyms:CAVIN1, CAVIN, CGL4, FKSG13, PTRF, cavin-1
Background:This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
UniProt Protein Function:PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription initiation complex

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; protein complex; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function:protein binding; rRNA primary transcript binding

Biological Process: regulation of transcription, DNA-dependent; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Lipodystrophy, Congenital Generalized, Type 4

NCBI Summary:This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]
UniProt Code:Q6NZI2
NCBI GenInfo Identifier:56749614
NCBI Gene ID:284119
NCBI Accession:Q6NZI2.1
UniProt Secondary Accession:Q6NZI2,O00535, Q6GMY1, Q96H74, Q9BT85, Q9HAP4, B2RAW7
UniProt Related Accession:Q6NZI2
Molecular Weight:390
NCBI Full Name:Polymerase I and transcript release factor
NCBI Synonym Full Names:polymerase I and transcript release factor
NCBI Official Symbol:PTRF  
NCBI Official Synonym Symbols:CGL4; CAVIN; CAVIN1; FKSG13; cavin-1  
NCBI Protein Information:polymerase I and transcript release factor; PTRF; TTF-I interacting peptide 12; RNA polymerase I and transcript release factor
UniProt Protein Name:Polymerase I and transcript release factor
UniProt Synonym Protein Names:Cavin-1
Protein Family:Polymerase I and transcript release factor
UniProt Gene Name:PTRF  
UniProt Entry Name:PTRF_HUMAN
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