Name: Anti-PRPF19 Antibody (CAB12590)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12590
Availability: OutOfStock

Antibody Name:	Anti-PRPF19 Antibody
Antibody SKU:	CAB12590
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, HeLa, Mouse heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AREA LATL KPQA GLIV PQAV PSSQ PSVV GAGE PMDL GELV GMTP EIIQ KLQD KATV LTTE RKKR GKTV PEEL VKPE ELSK YRQV ASHV GLHS ASIP GILA LDLC PSDT NKIL TGGA DKNV VVFD KSSE QILA TLKG HTKK VTSV VFHP SQDL VFSA SPDA TIRI WSVP NASC VQVV RAHE SAVT GLSL HATG DYLL SSSD DQYW AFSD IQTG RVLT KVTD ETSG CSLT CAQF HPDG LIFG TGTM DSQI KIWD LKER TNVA NFPG HSGP ITSI AFSE NGYY LATA ADDS SV
Gene ID:	27339
Uniprot:	Q9UMS4
Cellular Location:	Cytoplasm, Lipid droplet, Nucleus, cytoskeleton, nucleoplasm, spindle
Calculated MW:	55kDa
Observed MW:	55kDa

Synonyms:	PRPF19, NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4
Background:

UniProt Protein Function:	PRPF19: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the WD repeat PRP19 family.
UniProt Protein Details:	Protein type:RNA splicing; Spliceosome; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 11q12.2 Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; lipid particle; spindle; nuclear speck; nucleus Molecular Function:identical protein binding; protein binding; ligase activity Biological Process: nuclear mRNA splicing, via spliceosome; assembly of spliceosomal tri-snRNP; proteasomal protein catabolic process; positive regulation of nuclear mRNA splicing, via spliceosome; negative regulation of neuron differentiation; protein polyubiquitination; RNA splicing; lipid biosynthetic process; spliceosome assembly; gene expression; inner cell mass cell proliferation; generation of catalytic spliceosome for first transesterification step; double-strand break repair via nonhomologous end joining; positive regulation of astrocyte differentiation
NCBI Summary:	PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]
UniProt Code:	Q9UMS4
NCBI GenInfo Identifier:	55976619
NCBI Gene ID:	27339
NCBI Accession:	Q9UMS4.1
UniProt Related Accession:	Q9UMS4
Molecular Weight:	504
NCBI Full Name:	Pre-mRNA-processing factor 19
NCBI Synonym Full Names:	pre-mRNA processing factor 19
NCBI Official Symbol:	PRPF19
NCBI Official Synonym Symbols:	PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
NCBI Protein Information:	pre-mRNA-processing factor 19; psoralen 4; PRP19/PSO4 homolog; senescence evasion factor; nuclear matrix protein 200; PRP19/PSO4 pre-mRNA processing factor 19 homolog; nuclear matrix protein NMP200 related to splicing factor PRP19
UniProt Protein Name:	Pre-mRNA-processing factor 19
UniProt Synonym Protein Names:	Nuclear matrix protein 200; PRP19/PSO4 homolog; Senescence evasion factor
UniProt Gene Name:	PRPF19
UniProt Entry Name:	PRP19_HUMAN

	Western blot analysis of extracts of various cell lines, using PRPF19 antibody (CAB12590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (CAB12590) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (CAB12590) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (CAB12590) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (CAB12590) at dilution of 1:100 (40x lens).