Antibody Name:	Anti-Protein HIRA Antibody
Antibody SKU:	CAB8461
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human
Positive Samples:	293T, HepG2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AQII EREG WKTN MDFV GHRK AVTV VKFN PKIF KKKQ KNGS SAKP SCPY CCCA VGSK DRSL SVWL TCLK RPLV VIHE LFDK SIMD ISWT LNGL GILV CSMD GSVA FLDF SQDE LGDP LSEE EKSR IHQS TYGK SLAI MTEA QLST AVIE NPEM LKYQ RRQQ QQQL DQKS AATR EMGS ATSV AGVV NGES LEDI RKNL LKKQ VETR TADG RRRI TPLC IAQL DTGD FSTA FFNS IPLS GSLA GTML SSHS SPQL LPLD SSTP NSFG ASKP CTEP VVAA SARP AGDS VNKD SMNA TSTP AALS P
Gene ID:	7290
Uniprot:	P54198
Cellular Location:	Nucleus, PML body
Calculated MW:	89kDa/111kDa
Observed MW:	112kDa

Synonyms:

HIRA, DGCR1, TUP1, TUPLE1

Background:

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

UniProt Protein Function:	HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.
UniProt Protein Details:	Protein type:Transcription regulation; Cell development/differentiation; Cell cycle regulation Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: nuclear chromatin; nucleoplasm; nucleus; protein complex Molecular Function:protein binding; transcription corepressor activity; transcription factor activity Biological Process: anatomical structure morphogenesis; DNA replication-independent nucleosome assembly; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]
UniProt Code:	P54198
NCBI GenInfo Identifier:	88984228
NCBI Gene ID:	7290
NCBI Accession:	P54198.2
UniProt Secondary Accession:	P54198,Q05BU9, Q8IXN2,
UniProt Related Accession:	P54198
Molecular Weight:	89,299 Da
NCBI Full Name:	Protein HIRA
NCBI Synonym Full Names:	histone cell cycle regulator
NCBI Official Symbol:	HIRA
NCBI Official Synonym Symbols:	TUP1; DGCR1; TUPLE1
NCBI Protein Information:	protein HIRA
UniProt Protein Name:	Protein HIRA
UniProt Synonym Protein Names:	TUP1-like enhancer of split protein 1
Protein Family:	HIRA-interacting protein
UniProt Gene Name:	HIRA
UniProt Entry Name:	HIRA_HUMAN

	Western blot analysis of extracts of various cell lines, using HIRA antibody (CAB8461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and HIRA Rabbit pAb knockout (KO) 293T cells, using HIRA Rabbit pAb antibody (CAB8461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using HIRA antibody (CAB8461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using HIRA antibody (CAB8461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using HIRA antibody (CAB8461) at dilution of 1:100 (40x lens).