Epigenetics & Nuclear Signaling Antibodies 4

Anti-Protein HIRA Antibody (CAB8461)

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SKU:
CAB8461
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Synonyms:
TUP1, DGCR1, TUPLE1, RA
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] HIRA Antibody (CAB8461)

The [KO Validated] HIRA Antibody (CAB8461) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot and immunohistochemistry applications.The HIRA protein is essential for maintaining genome integrity and regulating gene expression, making it a key player in cellular processes such as development, DNA replication, and transcriptional regulation. Dysregulation of HIRA has been linked to various diseases, including cancer and developmental disorders, highlighting its importance in biological pathways.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] HIRA Antibody
SKU:CAB8461
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:AQII EREG WKTN MDFV GHRK AVTV VKFN PKIF KKKQ KNGS SAKP SCPY CCCA VGSK DRSL SVWL TCLK RPLV VIHE LFDK SIMD ISWT LNGL GILV CSMD GSVA FLDF SQDE LGDP LSEE EKSR IHQS TYGK SLAI MTEA QLST AVIE NPEM LKYQ RRQQ QQQL DQKS AATR EMGS ATSV AGVV NGES LEDI RKNL LKKQ VETR TADG RRRI TPLC IAQL DTGD FSTA FFNS IPLS GSLA GTML SSHS SPQL LPLD SSTP NSFG ASKP CTEP VVAA SARP AGDS VNKD SMNA TSTP AALS P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TUP1, DGCR1, TUPLE1, RA
Positive Sample:293T, HepG2, HeLa
Cellular Localization:Nucleus, Pml Body.
Calculated MW:112kDa
Observed MW:112kDa

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Purification MethodAffinity purification
Gene ID7290
RRIDAB_2769783
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (CAB8461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from wild type (WT) and HIRA knockout (KO) 293T cells, using [KO Validated] HIRA Rabbit pAb (CAB8461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] HIRA Rabbit pAb (CAB8461) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HIRA Rabbit pAb (CAB8461) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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