Antibody Name:	Anti-PRMT6 Antibody [KO Validated]
Antibody SKU:	CAB7814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PRMT6 (NP_060607.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, SKOV3, MCF7, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PRMT6 (NP_060607.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQP KKRK LESG GGGE GGEG TEEE DGAE REAA LERP RRTK RERD QLYY ECYS DVSV HEEM IADR VRTD AYRL GILR NWAA LRGK TVLD VGAG TGIL SIFC AQAG ARRV YAVE ASAI WQQA REVV RFNG LEDR VHVL PGPV ETVE LPEQ VDAI VSEW MGYG LLHE SMLS SVLH ARTK WLKE GGLL LPAS AELF IAPI SDQM LEWR LGFW SQVK QHYG VDMS CLEG FATR CLMG HSEI VVQG LSGE DVLA RPQR FAQL ELSR AGLE QELE AGVG GRFR CSCY GSAP MHGF AIWF QVTF PGGE SEKP LVLS TSPF HPAT HWKQ ALLY LNEP VQVE QDTD VSGE ITLL PSRD NPRR LRVL LRYK VGDQ EEKT KDFA MED
Gene ID:	55170
Uniprot:	Q96LA8
Cellular Location:	Nucleus
Calculated MW:	32kDa/41kDa
Observed MW:	42kDa

Synonyms:

PRMT6, HRMT1L6

Background:

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity.

UniProt Protein Function:	PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: nucleoplasm; nucleus; cytosol Molecular Function:histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]
UniProt Code:	Q96LA8
NCBI GenInfo Identifier:	20137409
NCBI Gene ID:	55170
NCBI Accession:	Q96LA8.1
UniProt Related Accession:	Q96LA8
Molecular Weight:
NCBI Full Name:	Protein arginine N-methyltransferase 6
NCBI Synonym Full Names:	protein arginine methyltransferase 6
NCBI Official Symbol:	PRMT6
NCBI Official Synonym Symbols:	HRMT1L6
NCBI Protein Information:	protein arginine N-methyltransferase 6
UniProt Protein Name:	Protein arginine N-methyltransferase 6
UniProt Synonym Protein Names:	Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6 (EC:2.1.1.125)
Protein Family:	Protein arginine N-methyltransferase
UniProt Gene Name:	PRMT6
UniProt Entry Name:	ANM6_HUMAN

	Western blot analysis of extracts of various cell lines, using PRMT6 antibody (CAB7814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and PRMT6 Rabbit pAb knockout (KO) 293T cells, using PRMT6 Rabbit pAb antibody (CAB7814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U2OS cells using PRMT6 antibody (CAB7814) at dilution of 1:100. Blue: DAPI for nuclear staining.