Epigenetics & Nuclear Signaling Antibodies 1
Anti-PRIM2 Antibody (CAB11633)
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- SKU:
- CAB11633
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PRIM2 Antibody |
| Antibody SKU: | CAB11633 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A375, H460, HeLa, HT-29, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEFS GRKW RKLR LAGD QRNA SYPH CLQF YLQP PSEN ISLI EFEN LAID RVKL LKSV ENLG VSYV KGTE QYQS KLES ELRK LKFS YREN LEDE YEPR RRDH ISHF ILRL AYCQ SEEL RRWF IQQE MDLL RFRF SILP KDKI QDFL KDSQ LQFE AISD EEKT LREQ EIVA SS |
| Gene ID: | 5558 |
| Uniprot: | P49643 |
| Cellular Location: | |
| Calculated MW: | 19kDa/58kDa |
| Observed MW: | 59kDa |
| Synonyms: | PRIM2, PRIM2A, p58 |
| Background: | This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. |
| UniProt Protein Function: | PRI2: synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The replication of DNA is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. |
| UniProt Protein Details: | Protein type:EC 2.7.7.-; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase Chromosomal Location of Human Ortholog: 6p11.2 Cellular Component: alpha DNA polymerase:primase complex; nucleoplasm Molecular Function:DNA primase activity; DNA-directed DNA polymerase activity; single-stranded DNA binding Biological Process: DNA replication initiation; G1/S transition of mitotic cell cycle; telomere maintenance via recombination |
| NCBI Summary: | This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014] |
| UniProt Code: | P49643 |
| NCBI GenInfo Identifier: | 51338777 |
| NCBI Gene ID: | 5558 |
| NCBI Accession: | P49643.2 |
| UniProt Secondary Accession: | P49643,Q53FJ8, Q6P1Q7, Q8WVL2, Q9H413, |
| UniProt Related Accession: | P49643 |
| Molecular Weight: | 59kDa |
| NCBI Full Name: | DNA primase large subunit |
| NCBI Synonym Full Names: | primase (DNA) subunit 2 |
| NCBI Official Symbol: | PRIM2 |
| NCBI Official Synonym Symbols: | p58; PRIM2A |
| NCBI Protein Information: | DNA primase large subunit |
| UniProt Protein Name: | DNA primase large subunit |
| UniProt Synonym Protein Names: | DNA primase 58 kDa subunit; p58 |
| UniProt Gene Name: | PRIM2 |
 | Western blot analysis of extracts of various cell lines, using PRIM2 antibody (CAB11633) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of mouse spleen, using PRIM2 antibody (CAB11633) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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