Cell Biology Antibodies 8

PPP4C Rabbit Polyclonal Antibody (CAB2109)

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SKU:
CAB2109
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
PP4, PPX, PP-X, PP4C, PPH3, PPP4, PPP4C
Research Use:
Cell Biology
Frequently bought together:

Description

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PPP4C Antibody (CAB2109)

The PPP4C Antibody (CAB2109) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for PPP4C in human samples, making it ideal for use in Western blot applications.PPP4C, a catalytic subunit of protein phosphatase 4, plays a crucial role in the regulation of signaling pathways that control cell growth and survival.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PPP4C Antibody
SKU:CAB2109
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAEI SDLD RQIE QLRR CELI KESE VKAL CAKA REIL VEES NVQR VDSP VTVC GDIH GQFY DLKE LFRV GGDV PETN YLFM GDFV DRGF YSVE TFLL LLAL KVRY PDRI TLIR GNHE SRQI TQVY GFYD ECLR KYGS VTVW RYCT EIFD YLSL SAII DGKI FCVH GGLS PSIQ TLDQ IRTI DRKQ EVPH DGPM CDLL WSDP EDTT GWGV SPRG AGYL FGSD VVAQ FNAA NDID MICR AHQL VMEG YKWH FNET VLTV WSAP NYCY RCGN VAAI LELD EHLQ KDFI IFEA APQE TRGI PSKK PVAD YFL
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PP4, PPX, PP-X, PP4C, PPH3, PPP4, PPP4C
Positive Sample:293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen
Cellular Localization:Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:35kDa
Observed MW:36kDa

Enables protein serine/threonine phosphatase activity. Involved in regulation of double-strand break repair via homologous recombination. Located in cytosol; nucleoplasm; and plasma membrane.

Purification MethodAffinity purification
Gene ID5531
RRIDAB_2764128
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PPP4C Rabbit pAb (CAB2109) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver using PPP4C Rabbit pAb (CAB2109) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using PPP4C Rabbit pAb (CAB2109) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg PPP4C antibody (CAB2109). Western blot was performed from the immunoprecipitate using PPP4C antibody (CAB2109) at a dilution of 1:1000.
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