Epigenetics & Nuclear Signaling Antibodies 2

Anti-PPP2R1A Antibody (CAB5799)

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SKU:
CAB5799
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PPP2R1A Antibody
Antibody SKU:CAB5799
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PPP2R1A (NP_055040.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HT-29, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PPP2R1A (NP_055040.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA DGDD SLYP IAVL IDEL RNED VQLR LNSI KKLS TIAL ALGV ERTR SELL PFLT DTIY DEDE VLLA LAEQ LGTF TTLV GGPE YVHC LLPP LESL ATVE ETVV RDKA VESL RAIS HEHS PSDL EAHF VPLV KRLA GGDW FTSR TSAC GLFS VCYP RVSS AVKA ELRQ YFRN LCSD DTPM VRRA AASK LGEF AKVL ELDN VKSE IIPM FSNL ASDE QDSV RLLA VEAC VNIA QLLP QEDL EALV MPTL RQAA EDKS WRVR YMVA DKFT EL
Gene ID:5518
Uniprot:P30153
Cellular Location:Chromosome, Cytoplasm, centromere
Calculated MW:65kDa
Observed MW:65KDa
Synonyms:PPP2R1A, MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A
Background:This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
UniProt Protein Function:PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.
UniProt Protein Details:

Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: microtubule cytoskeleton; protein phosphatase type 2A complex; mitochondrion; membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function:protein binding; protein heterodimerization activity; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; antigen binding

Biological Process: regulation of cell adhesion; second-messenger-mediated signaling; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; organelle organization and biogenesis; RNA splicing; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; chromosome segregation; response to organic substance; regulation of transcription, DNA-dependent; regulation of growth; regulation of cell differentiation; mRNA catabolic process, nonsense-mediated decay; negative regulation of tyrosine phosphorylation of Stat3 protein; protein complex assembly; gene expression; mitotic cell cycle; negative regulation of cell growth; G2/M transition of mitotic cell cycle; ceramide metabolic process; inactivation of MAPK activity; regulation of DNA replication

Disease: Mental Retardation, Autosomal Dominant 36

NCBI Summary:This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
UniProt Code:P30153
NCBI GenInfo Identifier:143811355
NCBI Gene ID:5518
NCBI Accession:P30153.4
UniProt Secondary Accession:P30153,Q13773, Q6ICQ3, Q96DH3,
UniProt Related Accession:P30153
Molecular Weight:589
NCBI Full Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NCBI Synonym Full Names:protein phosphatase 2, regulatory subunit A, alpha
NCBI Official Symbol:PPP2R1A  
NCBI Official Synonym Symbols:PR65A; PP2AAALPHA; PP2A-Aalpha  
NCBI Protein Information:serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform R1-alpha; PP2A subunit A isoform PR65-alpha; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Synonym Protein Names:Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha
UniProt Gene Name:PPP2R1A  
UniProt Entry Name:2AAA_HUMAN
Anti-PPP2R1A Antibody (CAB5799)Western blot analysis of extracts of various cell lines, using PPP2R1A Polyclonal Antibody (CAB5799) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-PPP2R1A Antibody (CAB5799)Immunofluorescence analysis of U2OS cells using PPP2R1A antibody (CAB5799). Blue: DAPI for nuclear staining.
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