Epigenetics & Nuclear Signaling Antibodies 3

POLR2J Rabbit Polyclonal Antibody (CAB1843)

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SKU:
CAB1843
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
RPB11, RPB11A, RPB11m, hRPB14, POLR2J1, POLR2J
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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POLR2J Antibody (CAB1843)

The POLR2J Antibody (CAB1843) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the POLR2J protein, it allows for the detection and analysis of this crucial transcription factor in a variety of cell types.The POLR2J protein is involved in the transcription of protein-coding genes and is crucial for the regulation of gene expression. Understanding its function and activity is essential for unraveling the complex mechanisms of transcription and gene regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:POLR2J Antibody
SKU:CAB1843
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MNAP PAFE SFLL FEGE KKIT INKD TKVP NACL FTIN KEDH TLGN IIKS QLLK DPQV LFAG YKVP HPLE HKII IRVQ TTPD YSPQ EAFT NAIT DLIS ELSL LEER FRVA IKDK QEGI E
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RPB11, RPB11A, RPB11m, hRPB14, POLR2J1, POLR2J
Positive Sample:K-562, Mouse thymus
Cellular Localization:Nucleus.
Calculated MW:13kDa
Observed MW:16kDa

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

Purification MethodAffinity purification
Gene ID5439
RRIDAB_2763879
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using POLR2J Rabbit pAb (CAB1843) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunofluorescence analysis of U2OS cells using POLR2J Rabbit pAb (CAB1843). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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