Cell Death Antibodies 2

PIDD Rabbit Polyclonal Antibody (CAB4831)

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SKU:
CAB4831
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
LRDD, PIDD, MRT75
Research Use:
Cell Death
Frequently bought together:

Description

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PIDD Antibody (CAB4831)

The PIDD Antibody (CAB4831) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PIDD protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and apoptosis research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PIDD Antibody
SKU:CAB4831
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAT VEGP ELEA AAAA GDAS EDSD AGSR ALPF LGGN RLSL DLYP GGCQ QLLH LCVQ QPLQ LLQV EFLR LSTH EDPQ LLEA TLAQ L
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LRDD, PIDD, MRT75
Positive Sample:U-87MG, 293T, K-562, Mouse brain, Mouse liver, Mouse heart, Rat brain
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:100kDa
Observed MW:100-110kDa

The protein encoded by this gene contains a leucine-rich repeat and a death domain. This protein has been shown to interact with other death domain proteins, such as Fas (TNFRSF6)-associated via death domain (FADD) and MAP-kinase activating death domain-containing protein (MADD), and thus may function as an adaptor protein in cell death-related signaling processes. The expression of the mouse counterpart of this gene has been found to be positively regulated by the tumor suppressor p53 and to induce cell apoptosis in response to DNA damage, which suggests a role for this gene as an effector of p53-dependent apoptosis. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID55367
RRIDAB_2765891
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PIDD Rabbit pAb (CAB4831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using PIDD Rabbit pAb (CAB4831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using PIDD Rabbit pAb (CAB4831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using PIDD Rabbit pAb (CAB4831) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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