Cell Death Antibodies 2

Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody (CABP0482)

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SKU:
CABP0482
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Synonyms:
S6K, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, Phospho-p70 S6 Kinase 1-S371
Research Use:
Cell Death
Frequently bought together:

Description

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Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody (CABP0482)

The Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody (CABP0482) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at serine 371 of p70S6K1 in human samples and is validated for use in Western blot applications.Phosphorylation of p70S6K1 at serine 371 plays a crucial role in the activation of this protein, leading to downstream signaling that promotes cell proliferation and survival. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways that regulate cellular processes such as growth and metabolism.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.

Product Name:Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody
SKU:CABP0482
Size:20μL, 100μL
Reactivity:Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:VDSP D
Tested Applications:WB ELISA
Recommended Dilution:
WB1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:S6K, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, Phospho-p70 S6 Kinase 1-S371
Positive Sample:NIH/3T3 treated with EGF
Cellular Localization:Cell Junction, Cytoplasm, Mitochondrion Outer Membrane, Mitochondrion, Nucleus, Synapse, Synaptosome.
Calculated MW:59kDa
Observed MW:70kDa

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Purification MethodAffinity purification
Gene ID6198
RRIDAB_2771383
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (CABP0482) at 1:400 dilution. NIH/3T3 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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