Phospho-Histone H3-T32 Rabbit Polyclonal Antibody (CABP0897)

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SKU:
CABP0897
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Synonyms:
H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A, Phospho-Histone H3-T32
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Phospho-Histone H3-T32 Antibody (CABP0897)

The Phospho-Histone H3-T32 Antibody (CABP0897) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets phosphorylated Histone H3 at Threonine 32, a key modification associated with mitosis and chromosome condensation. Developed using advanced technology, this antibody offers high specificity and sensitivity for detecting phosphorylated Histone H3 at T32 in a variety of samples. It has been rigorously validated for use in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry, providing reliable results for researchers investigating cell cycle progression, proliferation, and epigenetic modifications.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:Phospho-Histone H3-T32 Antibody
SKU:CABP0897
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PATG G
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A, Phospho-Histone H3-T32
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:Refertofigures

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification MethodAffinity purification
Gene ID8290 8350
RRIDAB_2863816
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T32 Rabbit pAb (CABP0897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using Phospho-Histone H3-T32 Rabbit pAb (CABP0897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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