Epigenetics & Nuclear Signaling Antibodies 5

Phospho-Histone H3-T45 Rabbit Polyclonal Antibody (CABP0898)

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SKU:
CABP0898
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Synonyms:
H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A, Phospho-Histone H3-T45
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Phospho-Histone H3-T45 Antibody (CABP0898)

The Phospho-Histone H3-T45 Antibody (CABP0898) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histone H3 is a core component of nucleosomes, the structural units of chromatin, and the phosphorylation of specific residues, such as threonine 45, plays a key role in regulation of gene expression and cell division.This antibody, produced in rabbits, is highly specific for detecting phosphorylated histone H3 at threonine 45 in human samples. It has been validated for use in Western blot applications, allowing for precise and sensitive detection of this specific histone modification.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:Phospho-Histone H3-T45 Antibody
SKU:CABP0898
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PGTV A
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A, Phospho-Histone H3-T45
Positive Sample:HeLa, HeLa treated with nocodazole, NIH/3T3, NIH/3T3 treated with nocodazole, C6, C6 treated with nocodazole
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification MethodAffinity purification
Gene ID8290 8350
RRIDAB_2863817
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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