Antibody Name:	Anti-PHGDH Antibody
Antibody SKU:	CAB10461
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, B cells, HeLa, SKOV3, Mouse brain, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEPP RDRA LVDH ENVI SCPH LGAS TKEA QSRC GEEI AVQF VDMV KGKS LTGV VNAQ ALTS AFSP HTKP WIGL AEAL GTLM RAWA GSPK GTIQ VITQ GTSL KNAG NCLS PAVI VGLL KEAS KQAD VNLV NAKL LVKE AGLN VTTS HSPA APGE QGFG ECLL AVAL AGAP YQAV GLVQ GTTP VLQG LNGA VFRP EVPL RRDL PLLL FRTQ TSDP AMLP TMIG LLAE AGVR LLSY QTSL VSDG ETWH VMGI SSLL PSLE AWKQ HVTE AFQF HF
Gene ID:	26227
Uniprot:	O43175
Cellular Location:
Calculated MW:	56kDa
Observed MW:	57kDa

Synonyms:

PHGDH, 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA

Background:

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

UniProt Protein Function:	PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
UniProt Protein Details:	Protein type:Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation Chromosomal Location of Human Ortholog: 1p12 Cellular Component: cytosol Molecular Function:electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1
NCBI Summary:	This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
UniProt Code:	O43175
NCBI GenInfo Identifier:	21264510
NCBI Gene ID:	26227
NCBI Accession:	O43175.4
UniProt Secondary Accession:	O43175,Q5SZU3, Q9BQ01, B2RD08,
UniProt Related Accession:	O43175
Molecular Weight:	533
NCBI Full Name:	D-3-phosphoglycerate dehydrogenase
NCBI Synonym Full Names:	phosphoglycerate dehydrogenase
NCBI Official Symbol:	PHGDH
NCBI Official Synonym Symbols:	NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
NCBI Protein Information:	D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113
UniProt Protein Name:	D-3-phosphoglycerate dehydrogenase
UniProt Gene Name:	PHGDH
UniProt Entry Name:	SERA_HUMAN

	Western blot analysis of extracts of various cell lines, using PHGDH antibody (CAB10461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using PHGDH antibody (CAB10461) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using PHGDH antibody (CAB10461) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using PHGDH antibody (CAB10461) at dilution of 1:100. Blue: DAPI for nuclear staining.