Epigenetics & Nuclear Signaling Antibodies 3

PELP1 Rabbit Polyclonal Antibody (CAB3189)

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SKU:
CAB3189
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
MNAR, P160, PELP1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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PELP1 Antibody (CAB3189)

The PELP1 Antibody (CAB3189) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By targeting the PELP1 protein, this antibody allows for the detection and analysis of PELP1 expression in different cell types, making it ideal for studies in cancer research and hormone signaling pathways.PELP1, a multifunctional protein involved in gene regulation and cell growth, is known to impact cancer cell proliferation and hormone receptor signaling.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PELP1 Antibody
SKU:CAB3189
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:EVEE GAPP PPTL PPAL PPPE SPPK VQPE PEPE PGLL LEVE EPGT EEER GADT APTL APEA LPSQ GEVE REGE SPAA GPPP QELV EEEP SAPP TLLE EETE DGSD KVQP PPET PAEE EMET ETEA EALQ EKEQ DDTA AMLA DFID CPPD DEKP PPPT EPDS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MNAR, P160, PELP1
Positive Sample:U-87MG, A-549, HeLa, NIH/3T3, Mouse liver, Rat uterus
Cellular Localization:Cytoplasm, Nucleus, Nucleoplasm.
Calculated MW:125kDa
Observed MW:160kDa

This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID27043
RRIDAB_2764975
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PELP1 Rabbit pAb (CAB3189) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of L929 cells using PELP1 Rabbit pAb (CAB3189) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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