Name: Anti-PBX1 Antibody (CAB0124)
Brand: Developmental Biology
SKU: CAB0124
Availability: OutOfStock

Antibody Name:	Anti-PBX1 Antibody
Antibody SKU:	CAB0124
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PBX1 (NP_002576.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	HeLa, SKOV3, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PBX1 (NP_002576.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EYFY SHLS NPYP SEEA KEEL AKKC GITV SQVS NWFG NKRI RYKK NIGK FQEE ANIY AAKT AVTA TNVS AHGS QANS PSTP NSAG SSSS FNMS NSGD LFMS V
Gene ID:	5087
Uniprot:	P40424
Cellular Location:	Nucleus
Calculated MW:	38kDa/46kDa
Observed MW:	50kDa

Synonyms:

PBX1

Background:

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PBX1: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1. Expressed in all tissues except in cells of the B and T lineage. Belongs to the TALE/PBX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding; Oncoprotein; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q23 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity Biological Process: spleen development; transcription, DNA-dependent; thymus development; embryonic hemopoiesis; somatic stem cell maintenance; adrenal gland development; negative regulation of transcription factor activity; embryonic skeletal development; anterior/posterior pattern formation; negative regulation of neuron differentiation; ureteric bud branching; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; steroid biosynthetic process; sex differentiation; proximal/distal pattern formation; embryonic limb morphogenesis
NCBI Summary:	This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
UniProt Code:	P40424
NCBI GenInfo Identifier:	730279
NCBI Gene ID:	5087
NCBI Accession:	P40424.1
UniProt Related Accession:	P40424
Molecular Weight:	38kDa; 46kDa
NCBI Full Name:	Pre-B-cell leukemia transcription factor 1
NCBI Synonym Full Names:	PBX homeobox 1
NCBI Official Symbol:	PBX1
NCBI Official Synonym Symbols:	CAKUHED
NCBI Protein Information:	pre-B-cell leukemia transcription factor 1
UniProt Protein Name:	Pre-B-cell leukemia transcription factor 1
UniProt Synonym Protein Names:	Homeobox protein PBX1; Homeobox protein PRL
Protein Family:	Pre-B-cell leukemia transcription factor
UniProt Gene Name:	PBX1
UniProt Entry Name:	PBX1_HUMAN

Western blot analysis of extracts of various cell lines, using PBX1 antibody (CAB0124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-PBX1 Antibody (CAB7567)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)