Developmental Biology
Anti-PAX2 Antibody (CAB3067)
- SKU:
- CAB3067
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-PAX2 Antibody |
| Antibody SKU: | CAB3067 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human PAX2 |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, Mouse spinal cord |
| Immunogen: | A synthetic peptide of human PAX2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5076 |
| Uniprot: | Q02962 |
| Cellular Location: | Nucleus |
| Calculated MW: | 41kDa/42kDa/44kDa |
| Observed MW: | 45kDa |
| Synonyms: | PAX2, FSGS7, PAPRS |
| Background: | PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | PAX2: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Interacts with ELGN3; the interaction targets PAX2 for destruction. Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10q24 Cellular Component: protein complex; lysosome; microtubule organizing center; nucleus Molecular Function:protein binding; DNA binding; superoxide-generating NADPH oxidase activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; negative regulation of cytolysis; optic nerve structural organization; positive regulation of transcription, DNA-dependent; cell fate determination; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; pronephros development; glial cell differentiation; vestibulocochlear nerve formation; visual perception; pancreas development; negative regulation of programmed cell death; protein kinase B signaling cascade; brain morphogenesis; mesonephros development; aging; response to nutrient levels; inner ear morphogenesis; camera-type eye development; transcription, DNA-dependent; optic cup morphogenesis involved in camera-type eye development; stem cell differentiation; optic nerve morphogenesis; axonogenesis; optic nerve development; ureteric bud branching; neural tube closure; mesodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; urogenital system development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis Disease: Papillorenal Syndrome; Renal Hypodysplasia/aplasia 1; Focal Segmental Glomerulosclerosis 7 |
| NCBI Summary: | PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q02962 |
| NCBI GenInfo Identifier: | 223590261 |
| NCBI Gene ID: | 5076 |
| NCBI Accession: | Q02962.4 |
| UniProt Secondary Accession: | Q02962,Q15105, Q15110, Q15837, Q5SZP2, Q5SZP3, |
| UniProt Related Accession: | Q02962 |
| Molecular Weight: | 393 |
| NCBI Full Name: | Paired box protein Pax-2 |
| NCBI Synonym Full Names: | paired box 2 |
| NCBI Official Symbol: | PAX2 |
| NCBI Official Synonym Symbols: | FSGS7; PAPRS |
| NCBI Protein Information: | paired box protein Pax-2; paired box homeotic gene 2 |
| UniProt Protein Name: | Paired box protein Pax-2 |
| Protein Family: | Paired box protein |
| UniProt Gene Name: | PAX2 |
| UniProt Entry Name: | PAX2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAX2 antibody (CAB3067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using PAX2 antibody (CAB3067). Blue: DAPI for nuclear staining. |
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