KO Validated Antibodies 1

Anti-PARP1 Antibody (CAB0942)[KO Validated]

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SKU:
CAB0942
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PARP1 Antibody [KO Validated]
Antibody SKU:CAB0942
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, RAW264.7, C6, Mouse testis, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DQQK VKKT AEAG GVTG KGQD GIGS KAEK TLGD FAAE YAKS NRST CKGC MEKI EKGQ VRLS KKMV DPEK PQLG MIDR WYHP GCFV KNRE ELGF RPEY SASQ LKGF SLLA TEDK EALK KQLP GVKS EGKR KGDE VDGV DEVA KKKS KKEK DKDS KLEK ALKA QNDL IWNI KDEL KKVC STND LKEL LIFN KQQV PSGE SAIL DRVA DGMV FGAL LPCE ECSG QLVF KSDA YYCT GDVT AWTK CMVK TQTP NRKE WVTP KEFR EISY LKKL KVKK QDRI FPPE TSAS VAAT PPPS TASA PAAV NSSA SADK PL
Gene ID:142
Uniprot:P09874
Cellular Location:Nucleus, nucleolus
Calculated MW:113kDa
Observed MW:100kDa, 110kDa, 125kDa
Synonyms:ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, PARP1, Cleaved PARP, PARP1
Background:This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
UniProt Protein Function:PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
UniProt Protein Details:

Protein type:DNA repair, damage; EC 2.4.2.30; Nuclear envelope; Nuclear receptor co-regulator; Nucleolus; Transferase

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: membrane; mitochondrion; nuclear chromosome, telomeric region; nuclear envelope; nucleolus; nucleoplasm; nucleus; protein complex; transcription factor complex

Molecular Function:DNA ligase (ATP) activity; enzyme binding; identical protein binding; NAD+ ADP-ribosyltransferase activity; protein binding; protein kinase binding; protein N-terminus binding; RNA binding; transcription factor binding

Biological Process: cellular response to insulin stimulus; DNA ligation during DNA repair; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; lagging strand elongation; macrophage differentiation; mitochondrial DNA metabolic process; mitochondrial DNA repair; mitochondrion organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; peptidyl-serine ADP-ribosylation; protein amino acid ADP-ribosylation; regulation of catalytic activity; response to DNA damage stimulus; telomere maintenance; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
UniProt Code:P09874
NCBI GenInfo Identifier:130781
NCBI Gene ID:142
NCBI Accession:P09874.4
UniProt Secondary Accession:P09874,Q8IUZ9, B1ANJ4,
UniProt Related Accession:P09874
Molecular Weight:113kDa
NCBI Full Name:Poly
NCBI Synonym Full Names:poly(ADP-ribose) polymerase 1
NCBI Official Symbol:PARP1  
NCBI Official Synonym Symbols:PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1  
NCBI Protein Information:poly [ADP-ribose] polymerase 1
UniProt Protein Name:Poly [ADP-ribose] polymerase 1
UniProt Synonym Protein Names:ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
Protein Family:Poly [ADP-ribose] polymerase
UniProt Gene Name:PARP1  
Anti-PARP1 Antibody [KO Validated] (CAB0942)Western blot analysis of extracts of various cell lines, using PARP1 antibody (CAB0942) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-PARP1 Antibody [KO Validated] (CAB0942)Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody (CAB0942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PARP1 Antibody [KO Validated] (CAB0942)Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (CAB0942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-PARP1 Antibody [KO Validated] (CAB0942)Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Rabbit pAb (CAB0942) at dilution of 1:200 (40x lens).
Anti-PARP1 Antibody [KO Validated] (CAB0942)Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Polyclonal Antibody (CAB0942) at dilution of 1:200. Blue: DAPI for nuclear staining.
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