Cell Biology Antibodies 18

Pan Mono-Methyl lysine Rabbit Polyclonal Antibody (CAB18293)

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SKU:
CAB18293
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Research Use:
Cell Biology
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Description

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Pan Mono-Methyl lysine Antibody (CAB18293)

The Pan Mono-Methyl lysine Antibody (CAB18293) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting mono-methylated lysine residues in various protein samples, with a particular affinity for human samples.The antibody is validated for use in a variety of applications, including Western blotting, immunoprecipitation, and immunofluorescence, allowing for the detection and analysis of mono-methylated lysine proteins in different cell types and tissues. Its versatility makes it ideal for studies in epigenetics, chromatin remodeling, and gene expression regulation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Pan Mono-Methyl lysine Antibody
SKU:CAB18293
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Observed MW:17-90kDa

Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).

Purification MethodAffinity purification
RRIDAB_2862066
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (CAB18293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of various lysates using Pan Mono-Methyl lysine Rabbit pAb (CAB18293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
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