 | Western blot analysis of extracts of various cell lines, using Pan DiMethyl-lysine antibody (CAB5870) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (CAB5870) at 1:1000 dilution. |
 | Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |