KO Validated Antibodies 1

[KO Validated] p38 MAPK Rabbit Polyclonal Antibody (CAB14401)

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SKU:
CAB14401
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, p38 MAPK
Research Use:
Cell Death
Frequently bought together:

Description

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[KO Validated] p38 MAPK Antibody (CAB14401)

The [KO Validated] p38 MAPK Antibody (CAB14401) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting MAPK14 protein in human samples, making it an excellent choice for Western blotting applications.MAPK14, also known as p38 MAPK, is involved in regulating inflammatory responses, stress signaling, and cell survival pathways. Dysregulation of MAPK14 activity has been linked to numerous diseases, including cancer, inflammatory disorders, and neurodegenerative conditions.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] p38 MAPK Antibody
SKU:CAB14401
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SLTQ MPKM NFAN VFIG ANPL AVDL LEKM LVLD SDKR ITAA QALA HAYF AQYH DPDD EPVA DPYD QSFE SRDL LIDE WKSL TYDE VISF VPPP LDQE EMES
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, p38 MAPK
Positive Sample:HeLa, BxPC-3, Mouse testis, Rat kidney
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:41kDa
Observed MW:41kDa

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Purification MethodAffinity purification
Gene ID1432
RRIDAB_2761271
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using p38 MAPK Rabbit pAb (CAB14401) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using p38 MAPK Rabbit pAb (CAB14401) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using p38 MAPK Rabbit pAb (CAB14401) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of U-87MG cells, using 3 μg p38 MAPK antibody (CAB14401). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (CAB14401) at a dilution of 1:1000.
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