The OTX1 Monoclonal Antibody (CAB19737) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, specifically binds to the OTX1 protein in human samples, making it suitable for use in Western blotting and immunohistochemistry applications.OTX1 is known for its role in the regulation of gene expression during embryonic development, making it a key player in neurogenesis and organogenesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
OTX1 Monoclonal Antibody
SKU:
CAB19737
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2264
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
37kDa
Observed MW:
37kDa
This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mouse is required for proper brain and sensory organ development and can cause epilepsy. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5013
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OTX1 Rabbit mAb (CAB19737) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
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