Developmental Biology
Anti-ODF2 Antibody (CAB15630)
- SKU:
- CAB15630
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-ODF2 Antibody |
| Antibody SKU: | CAB15630 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 630-829 of human ODF2 (NP_702911.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat, 293T, Mouse brain, Mouse lung, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 630-829 of human ODF2 (NP_702911.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IISD LRSR IEHQ GDKL EMAR EKHQ ASQK ENKQ LSLK VDEL ERKL EATS AQNI EFLQ VIAK REEA IHQS QLRL EEKT RECG TLAR QLES AIED ARRQ VEQT KEHA LSKE RAAQ NKIL DLET QLSR TKTE LSQL RRSR DDAD RRYQ SRLQ DLKD RLEQ SEST NRSM QNYV QFLK SSYA NVFG DGPY STFL TSSP IRSR SPPA |
| Gene ID: | 4957 |
| Uniprot: | Q5BJF6 |
| Cellular Location: | |
| Calculated MW: | 66kDa/73kDa/75kDa/80kDa/88kDa/93kDa/95kDa |
| Observed MW: | 80kDa |
| Synonyms: | ODF2, CT134, ODF2/1, ODF2/2, ODF84 |
| Background: | The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. |
| NCBI Summary: | The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q5BJF6 |
| NCBI GenInfo Identifier: | 24430181 |
| NCBI Gene ID: | 4957 |
| NCBI Accession: | NP_002531 |
| UniProt Related Accession: | Q5BJF6 |
| Molecular Weight: | 89kDa |
| NCBI Full Name: | outer dense fiber protein 2 isoform 9 |
| NCBI Synonym Full Names: | outer dense fiber of sperm tails 2 |
| NCBI Official Symbol: | ODF2 |
| NCBI Official Synonym Symbols: | CT134; ODF84; ODF2/1; ODF2/2 |
| NCBI Protein Information: | outer dense fiber protein 2 |
| UniProt Protein Name: | Outer dense fiber protein 2 |
| UniProt Synonym Protein Names: | Cenexin; Outer dense fiber of sperm tails protein 2 |
| Protein Family: | Outer dense fiber protein |
| UniProt Gene Name: | ODF2 |
| UniProt Entry Name: | ODFP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ODF2 antibody (CAB15630) at dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (CAB15630) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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