Autophagy Antibodies

NRBF2 Rabbit Polyclonal Antibody (CAB6462)

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SKU:
CAB6462
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
Reactivity:
Human, Mouse, Rat
Synonyms:
COPR, COPR1, COPR2, NRBF-2, F2
Research Use:
Autophagy
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Description

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NRBF2 Antibody (CAB6462)

The NRBF2 Antibody (CAB6462) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is specifically designed for detecting NRBF2 in human samples through Western blot applications. Its high reactivity and specificity make it an ideal choice for investigating the role of NRBF2 in various cellular processes.NRBF2, also known as Nuclear Receptor Binding Factor 2, is a key player in the autophagy pathway, which is essential for maintaining cellular homeostasis and responding to stress.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NRBF2 Antibody
SKU:CAB6462
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MEVM EGPL NLAH QQSR RADR LLAA GKYE EAIS CHKK AAAY LSEA MKLT QSEQ AHLS LELQ RDSH MKQL LLIQ ERWK RAQR EERL KAQQ NTDK DAAA HLQT SHKP SAED AEGQ SPLS QKYS PSTE KCLP EIQG IFDR DPDT LLYL LQQK SEPA EPCI GSKA PKDD KTII EEQA TKIA DLKR HVEF LVAE NERL RKEN KQLK AEKA RLLK GPIE KELD VDAD FVET SELW SLPP HAET ATAS STWQ KFAA NTGK AKDI PIPN LPPL DFPS PELP LMEL SEDI LKGF MNN
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:COPR, COPR1, COPR2, NRBF-2, F2
Positive Sample:HepG2
Cellular Localization:Cytoplasm, Cytoplasmic Vesicle, Nucleus, Autophagosome.
Calculated MW:32kDa
Observed MW:38kDa

Involved in autophagy. Located in cytoplasm. Colocalizes with phosphatidylinositol 3-kinase complex, class III.

Purification MethodAffinity purification
Gene ID29982
RRIDAB_2767064
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using NRBF2 Rabbit pAb (CAB6462) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from HepG2 cells, using [KO Validated] NRBF2 Rabbit pAb (CAB6462) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal using [KO Validated] NRBF2 Rabbit pAb (CAB6462) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using [KO Validated] NRBF2 Rabbit pAb (CAB6462) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using [KO Validated] NRBF2 Rabbit pAb (CAB6462) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of C6 cells using NRBF2 Rabbit pAb (CAB6462) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using NRBF2 Rabbit pAb (CAB6462) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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