Cell Biology Antibodies 10

NPHP1 Rabbit Polyclonal Antibody (CAB6674)

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SKU:
CAB6674
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
NPH1, JBTS4, SLSN1, NPHP1
Research Use:
Cell Biology
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Description

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NPHP1 Antibody (CAB6674)

The NPHP1 Antibody (CAB6674) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for immunofluorescence and immunohistochemistry applications.Nephrocystin-1 is a key player in the development and maintenance of primary cilia, cellular appendages with crucial roles in signaling pathways and cell cycle regulation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NPHP1 Antibody
SKU:CAB6674
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PDLG ILFE LGIS YIRN STGE RGEL SCGW VFLK LFDA SGVP IPAK TYEL FLNG GTPY EKGI EVDP SISR RAHG SVFY QIMT MRRQ PQLL VKLR SLNR RSRN VLSL LPET LIGN MCSI HLLI FYRQ ILGD VLLK DRMS LQST DLIS HPML ATFP MLLE QPDV MDAL RSSW AGKE STLK RSEK RDKE FLKS TFLL VYHD CVLP LLHS TRLP PFRW AEEE TETA RWKV ITDF LKQN QENQ GALQ ALLS PDGV HEPF DLSE QTYD FLGE MRKN AV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NPH1, JBTS4, SLSN1, NPHP1
Positive Sample:PC-3, Mouse pancreas, Rat skeletal muscle, Rat brain
Cellular Localization:Cell Junction, Cell Projection, Cytoplasm, Adherens Junction, Cilium, Cilium Axoneme, Cytoskeleton, Tight Junction.
Calculated MW:83kDa
Observed MW:83kDa

This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell and cell-matrix adhesion signaling, likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1, a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1, also referred to as juvenile nephronophthisis with Leber amaurosis, which is characterized by kidney and eye disease, and with Joubert syndrome type 4, which is characterized by cerebellar ataxia, oculomotor apraxia, psychomotor delay and neonatal breathing abnormalities, sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID4867
RRIDAB_2767259
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse pancreas, using NPHP1 Rabbit pAb (CAB6674) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of various lysates using NPHP1 Rabbit pAb (CAB6674) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis using NPHP1 Rabbit pAb (CAB6674) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using NPHP1 Rabbit pAb (CAB6674) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using NPHP1 Rabbit pAb (CAB6674) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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