Developmental Biology
Anti-NOTCH4 Antibody (CAB8303)
- SKU:
- CAB8303
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | NOTCH4 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8303 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPEA RHKA TPGR EAGP FPRA RTVS VSVP PHGG GALP RCRT LSAG AGPR GGGA CLQA RTWS VDLA ARGG GAYS HCRS LSGV GAGG GPTP RGRR FSAG MRGP RPNP AIMR GRYG VAAG RGGR VSTD DWPC DWVA LGAC GSAS NIPI PPPC LTPS PERG SPQL DCGP PALQ EMPI NQGG EGKK |
| Gene ID: | 4855 |
| Uniprot: | Q99466 |
| Cellular Location: | Cell membrane, Nucleus, Single-pass type I membrane protein |
| Calculated MW: | 39kDa/61kDa/209kDa |
| Observed MW: | 88kDa |
| Synonyms: | NOTCH4, INT3, notch 4 |
| Background: | This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
| UniProt Protein Function: | NOTCH4: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cell surface; cytoplasmic vesicle; cytosol; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral to plasma membrane; nucleoplasm; nucleus; plasma membrane Molecular Function:calcium ion binding; Notch binding; protein binding; protein heterodimerization activity; receptor activity Biological Process: activation of Notch receptor target transcription factor; cell differentiation; cell fate determination; embryonic development; endothelial cell morphogenesis; gene expression; hemopoiesis; mammary gland development; morphogenesis of a branching structure; negative regulation of cell differentiation; negative regulation of endothelial cell differentiation; negative regulation of Notch signaling pathway; Notch receptor processing; Notch signaling pathway; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of transcription, DNA-dependent; regulation of protein localization; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| UniProt Code: | Q99466 |
| NCBI GenInfo Identifier: | 20139103 |
| NCBI Gene ID: | 4855 |
| NCBI Accession: | Q99466.2 |
| UniProt Secondary Accession: | Q99466,O00306, Q5SSY7, Q99458, Q99940, Q9H3S8, Q9UII9 Q9UIJ0, B0V183, B0V1X5, |
| UniProt Related Accession: | Q99466 |
| Molecular Weight: | 39,617 Da |
| NCBI Full Name: | Neurogenic locus notch homolog protein 4 |
| NCBI Synonym Full Names: | notch 4 |
| NCBI Official Symbol: | NOTCH4 |
| NCBI Official Synonym Symbols: | INT3 |
| NCBI Protein Information: | neurogenic locus notch homolog protein 4 |
| UniProt Protein Name: | Neurogenic locus notch homolog protein 4 |
| Protein Family: | Neurogenic locus notch homolog protein |
| UniProt Gene Name: | NOTCH4 |
| UniProt Entry Name: | NOTC4_HUMAN |
 | Western blot analysis of extracts of Mouse kidney, using NOTCH4 antibody (CAB8303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using NOTCH4 Rabbit pAb (CAB8303) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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