Antibody Name:	Anti-NLRP1 Antibody
Antibody SKU:	CAB16212
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human NLRP1 (NP_001028225.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SH-SY5Y

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human NLRP1 (NP_001028225.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TGEM SNST SSLK RQRL GSER AASH VAQA NLKL LDVS KIFP IAEI AGKS HEES SPEV VPVE LLCV PSPA SQGD LHTK PLGT DDDF WGPT GPVA TEVV DKEK N
Gene ID:	22861
Uniprot:	Q9C000
Cellular Location:
Calculated MW:	46kDa/83kDa/154-165kDa
Observed MW:	160KDa

Synonyms:

NLRP1, AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1

Background:

This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

UniProt Protein Function:	As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PubMed:22665479, PubMed:17418785). May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner (PubMed:18511561). Contrary to its mouse ortholog, not activated by Bacillus anthracis lethal toxin (PubMed:19651869). It is unclear whether isoform 2 is involved in inflammasome formation. It is not cleaved within the FIIND domain, does not assemble into specks, nor promote IL1B release (PubMed:22665479). However, in an vitro cell-free system, it has been shown to be activated by MDP (PubMed:17349957). Binds ATP (PubMed:11113115, PubMed:15212762).
NCBI Summary:	This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9C000
NCBI GenInfo Identifier:	17380146
NCBI Gene ID:	22861
NCBI Accession:	Q9C000.1
UniProt Secondary Accession:	Q9C000,Q9BZZ8, Q9BZZ9, Q9H5Z7, Q9H5Z8, Q9HAV8, Q9UFT4 Q9Y2E0, E9PE50, I6L9D9,
UniProt Related Accession:	Q9C000
Molecular Weight:	46,068 Da
NCBI Full Name:	NACHT, LRR and PYD domains-containing protein 1
NCBI Synonym Full Names:	NLR family pyrin domain containing 1
NCBI Official Symbol:	NLRP1
NCBI Official Synonym Symbols:	NAC; MSPC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
NCBI Protein Information:	NACHT, LRR and PYD domains-containing protein 1
UniProt Protein Name:	NACHT, LRR and PYD domains-containing protein 1
UniProt Synonym Protein Names:	Caspase recruitment domain-containing protein 7; Death effector filament-forming ced-4-like apoptosis protein; Nucleotide-binding domain and caspase recruitment domain
Protein Family:	NACHT, LRR and PYD domains-containing protein
UniProt Gene Name:	NLRP1

	Western blot analysis of extracts of various cell lines, using NLRP1 antibody (CAB16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using NLRP1 Rabbit pAb (CAB16212) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using NLRP1 Rabbit pAb (CAB16212) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using NLRP1 Rabbit pAb (CAB16212) at dilution of 1:100 (40x lens).