Epigenetics & Nuclear Signaling Antibodies 4

NFATC3 Rabbit Polyclonal Antibody (CAB6666)

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SKU:
CAB6666
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
NFAT4, NFATX, NF-AT4c, n339260, NFATC3
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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NFATC3 Antibody (CAB6666)

The NFATC3 Antibody (CAB6666) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the NFATC3 protein, this antibody enables precise detection and analysis in various cell types, making it an essential asset for studies in immunology and cancer research.NFATC3 is a crucial player in immune responses, serving as a key mediator in the activation of T cells and B cells.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NFATC3 Antibody
SKU:CAB6666
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MTTA NCGA HDEL DFKL VFGE DGAP APPP PGSR PADL EPDD CASI YIFN VDPP PSTL TTPL CLPH HGLP SHSS VLSP SFQL QSHK NYEG TCEI PESK YSPL GGPK PFEC PSIQ ITSI SPNC HQEL DAHE DDLQ INDP EREF LERP SRDH LYLP LEPS YRES SLSP SPAS SISS RSWF SDAS SCES LSHI YDDV DSEL NEAA ARFT LGSP LTSP GGSP GGCP GEET WHQQ YGLG HSLS PRQS PCHS PRSS VTDE NWLS PRPA SGPS SRPT SPCG KRRH SSAE VCYA GSLS PH
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NFAT4, NFATX, NF-AT4c, n339260, NFATC3
Positive Sample:Jurkat, Mouse thymus
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:116kDa
Observed MW:150kDa

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.

Purification MethodAffinity purification
Gene ID4775
RRIDAB_2767253
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NFATC3 Rabbit pAb (CAB6666) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFATC3 Rabbit pAb (CAB6666) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of K-562 cells using NFATC3 Rabbit pAb (CAB6666) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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