KO Validated Antibodies 1

Anti-NDUFC2 Antibody (CAB15073)[KO Validated]

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SKU:
CAB15073
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NDUFC2 Antibody [KO Validated]
Antibody SKU:CAB15073
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, OVCAR3, HeLa, A-549, mouse stomach, mouse brain, mouse heart, rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIAR RNPE PLRF LPDE ARSL PPPK LTDP RLLY IGFL GYCS GLID NLIR RRPI ATAG LHRQ LLYI TAFF FAGY YLVK REDY LYAV RDRE MFGY MKLH PEDF
Gene ID:4718
Uniprot:O95298
Cellular Location:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:10kDa/11kDa/13kDa/14kDa
Observed MW:14kDa
Synonyms:NDUFC2, B14.5b, CI-B14.5b, HLC-1, NADHDH2
Background:
UniProt Protein Function:NDUFC2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFC2 subunit family.
UniProt Protein Details:

Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly

UniProt Code:O95298
NCBI GenInfo Identifier:6093464
NCBI Gene ID:4718
NCBI Accession:O95298.1
UniProt Secondary Accession:O95298,Q549M5, Q6FIH8, Q9UBJ9, E9PNU8, E9PRB2,
UniProt Related Accession:O95298
Molecular Weight:13,408 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit C2
NCBI Official Symbol:NDUFC2  
NCBI Official Synonym Symbols:HLC-1; B14.5b; NADHDH2; CI-B14.5b  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 subunit C2
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 subunit C2
UniProt Synonym Protein Names:Complex I-B14.5b; CI-B14.5b; Human lung cancer oncogene 1 protein; HLC-1; NADH-ubiquinone oxidoreductase subunit B14.5b
Protein Family:NADH dehydrogenase
UniProt Gene Name:NDUFC2  
UniProt Entry Name:NDUC2_HUMAN
Anti-NDUFC2 Antibody [KO Validated] (CAB15073)Western blot analysis of extracts of various cell lines, using NDUFC2 antibody (CAB15073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-NDUFC2 Antibody [KO Validated] (CAB15073)Western blot analysis of extracts from normal (control) and NDUFC2 knockout (KO) HeLa cells, using NDUFC2 antibody (CAB15073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
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