KO Validated Antibodies 2

Anti-NDUFB5 Antibody (CAB19953)[KO Validated]

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SKU:
CAB19953
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NDUFB5 Antibody [KO Validated]
Antibody SKU:CAB19953
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human NDUFB5.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human NDUFB5.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4711
Uniprot:O43674
Cellular Location:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:15kDa/21kDa
Observed MW:16KDa
Anti-NDUFB5 Antibody [KO Validated] (CAB19953)Western blot analysis of extracts from normal (control) and NDUFB5 knockout (KO) HeLa cells, using NDUFB5 antibody (CAB19953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:NDUFB5, CISGDH, SGDH
Background:The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
NCBI Summary:The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:O43674
NCBI GenInfo Identifier:316660086
NCBI Gene ID:4711
NCBI Accession:NP_001186886.1
UniProt Secondary Accession:O43674,Q561V6,
UniProt Related Accession:O43674
Molecular Weight:20
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit B5
NCBI Official Symbol:NDUFB5  
NCBI Official Synonym Symbols:SGDH; CISGDH  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
UniProt Synonym Protein Names:Complex I-SGDH; CI-SGDH; NADH-ubiquinone oxidoreductase SGDH subunit
UniProt Gene Name:NDUFB5  
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