KO Validated Antibodies 2

Anti-NDUFA4 Antibody (CAB19893)[KO Validated]

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SKU:
CAB19893
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NDUFA4 Antibody [KO Validated]
Antibody SKU:CAB19893
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human NDUFA4.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human NDUFA4.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4697
Uniprot:O00483
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:9kDa
Observed MW:9KDa
Anti-NDUFA4 Antibody [KO Validated] (CAB19893)Western blot analysis of extracts from normal (control) and NDUFA4 knockout (KO) 293T cells, using NDUFA4 antibody (CAB19893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:NDUFA4, CI-9k, CI-MLRQ, MLRQ
Background:The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
UniProt Protein Function:NDUFA4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA4 subunit family.
UniProt Protein Details:

Protein type:Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Mitochondrial; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrial respiratory chain complex IV; mitochondrion

Molecular Function:cytochrome-c oxidase activity; protein binding; protein complex binding

Biological Process: mitochondrial electron transport, cytochrome c to oxygen

NCBI Summary:The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
UniProt Code:O00483
NCBI GenInfo Identifier:2499333
NCBI Gene ID:4697
NCBI Accession:O00483.1
UniProt Secondary Accession:O00483,Q6FHN5, A4D109,
UniProt Related Accession:O00483
Molecular Weight:9,370 Da
NCBI Full Name:Cytochrome c oxidase subunit NDUFA4
NCBI Synonym Full Names:NDUFA4, mitochondrial complex associated
NCBI Official Symbol:NDUFA4  
NCBI Official Synonym Symbols:MLRQ; CI-9k; CI-MLRQ  
NCBI Protein Information:cytochrome c oxidase subunit NDUFA4
UniProt Protein Name:Cytochrome c oxidase subunit NDUFA4
UniProt Synonym Protein Names:Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit
Protein Family:Cytochrome c oxidase
UniProt Gene Name:NDUFA4  
UniProt Entry Name:NDUA4_HUMAN
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