Cell Death Antibodies 2

Anti-NDUFA13 Antibody (CAB5412)

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SKU:
CAB5412
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-NDUFA13 Antibody
Antibody SKU:CAB5412
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 52-144 of human NDUFA13 (NP_057049.5).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, K-562, 293T, 3T3L1, M21, SK-Hep1, Mouse kidney, Mouse heart, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 52-144 of human NDUFA13 (NP_057049.5).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KWNR ERRR LQIE DFEA RIAL LPLL QAET DRRT LQML RENL EEEA IIMK DVPD WKVG ESVF HTTR WVPP LIGE LYGL RTTE EALH ASHG FMWY T
Gene ID:51079
Uniprot:Q9P0J0
Cellular Location:Matrix side, Mitochondrion inner membrane, Nucleus, Single-pass membrane protein
Calculated MW:16kDa/24kDa
Observed MW:17kDa
Synonyms:NDUFA13, B16.6, CDA016, CGI-39, GRIM-19, GRIM19
Background:This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.
UniProt Protein Function:GRIM-19: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC). Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Belongs to the complex I NDUFA13 subunit family.
UniProt Protein Details:

Protein type:Oxidoreductase; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; mitochondrion; mitochondrial membrane; mitochondrial inner membrane; cytoplasm; integral to membrane; mitochondrial respiratory chain; mitochondrial respiratory chain complex I

Molecular Function:protein binding; NADH dehydrogenase (ubiquinone) activity; ATP binding; NADH dehydrogenase activity

Biological Process: cellular metabolic process; positive regulation of protein catabolic process; positive regulation of caspase activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; protein import into mitochondrial inner membrane

Disease: Thyroid Carcinoma, Hurthle Cell

NCBI Summary:This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined. [provided by RefSeq, Oct 2009]
UniProt Code:Q9P0J0
NCBI GenInfo Identifier:20139242
NCBI Gene ID:51079
NCBI Accession:Q9P0J0.3
UniProt Related Accession:Q9P0J0
Molecular Weight:
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit A13
NCBI Official Symbol:NDUFA13  
NCBI Official Synonym Symbols:B16.6; CDA016; CGI-39; GRIM19; GRIM-19; MC1DN28  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
UniProt Synonym Protein Names:Cell death regulatory protein GRIM-19; Complex I-B16.6; CI-B16.6; Gene associated with retinoic and interferon-induced mortality 19 protein; GRIM-19; Gene associated with retinoic and IFN-induced mortality 19 protein; NADH-ubiquinone oxidoreductase B16.6 subunit
UniProt Gene Name:NDUFA13  
UniProt Entry Name:NDUAD_HUMAN
Anti-NDUFA13 Antibody (CAB5412)Western blot analysis of extracts of various cell lines, using NDUFA13 antibody (CAB5412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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