Cell Biology Antibodies 12

Anti-NDUFA1 Antibody (CAB8326)

(No reviews yet) Write a Review
SKU:
CAB8326
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NDUFA1 Antibody
Antibody SKU:CAB8326
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human NDUFA1 (NP_004532.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, Mouse kidney, Mouse heart, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human NDUFA1 (NP_004532.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWFE ILPG LSVM GVCL LIPG LATA YIHR FTNG GKEK RVAH FGYH WSLM ERDR RISG VDRY YVSK GLEN ID
Gene ID:4694
Uniprot:O15239
Cellular Location:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:8kDa
Observed MW:14kDa
Synonyms:NDUFA1, CI-MWFE, MWFE, ZNF183
Background:The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the 'hydrophobic protein' (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
UniProt Protein Function:NDUFA1: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA1 subunit family.
UniProt Protein Details:

Protein type:Oxidoreductase; Mitochondrial; EC 1.6.5.3; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial respiratory chain complex I

Molecular Function:NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency

NCBI Summary:The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]
UniProt Code:O15239
NCBI GenInfo Identifier:3334271
NCBI Gene ID:4694
NCBI Accession:O15239.1
UniProt Related Accession:O15239
Molecular Weight:8,072 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
NCBI Official Symbol:NDUFA1  
NCBI Official Synonym Symbols:MWFE; ZNF183; CI-MWFE  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; complex I-MWFE; type I dehydrogenase; complex I MWFE subunit; NADH oxidoreductase subunit MWFE; NADH:ubiquinone oxidoreductase (com
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
UniProt Synonym Protein Names:Complex I-MWFE; CI-MWFE; NADH-ubiquinone oxidoreductase MWFE subunit
UniProt Gene Name:NDUFA1  
UniProt Entry Name:NDUA1_HUMAN
Anti-NDUFA1 Polyclonal Antibody (CAB8326)Western blot analysis of extracts of various cell lines, using NDUFA1 antibody (CAB8326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products

Anti-NDUFA1 Antibody (CAB20940)

Anti-NDUFA1 Antibody (CAB20940)

OverviewAntibody Name:Anti-NDUFA1 Antibody (CAB20940)Antibody SKU:CAB20940Antibody Size:50µL, 100µLApplication:Western blotting, Immunohistochemistry