Anti-NDUFA1 Antibody (CAB20940)
- SKU:
- CAB20940
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant - Arabidopsis
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-NDUFA1 Antibody (CAB20940) |
| Antibody SKU: | CAB20940 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human NDUFA1. |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | K-562, U2OS |
| Immunogen: | Recombinant protein of Human NDUFA1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Matrix side, Mitochondrion inner membrane, Single-pass membrane protein |
| Calculated MW: | 8kDa |
| Observed MW: | 10KDa |
| Synonyms: | NDUFA1, CI-MWFE, MWFE, ZNF183 |
| Background: | The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the 'hydrophobic protein' (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. |
 | Immunohistochemistry of paraffin-embedded human liver using NDUFA1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human small intestine using NDUFA1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using NDUFA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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